EGE UNIVERSITY Lifelong Learning Programme

Description of Individual Course Units

Course Unit Code	Course Unit Title	Type of Course Unit	Year of Study	Semester	Number of ECTS Credits
9105025402014	Molecular Methods in Microbial Ecology	Elective	1	2	8

Level of Course Unit

Second Cycle

Language of Instruction

Turkish

Objectives of the Course

The purpose of this course is to get the latest generation of tools in molecular microbial ecology.

Name of Lecturer(s)

Prof.Dr. Güven Özdemir

Learning Outcomes

1	Çevresel örneklerde bulunan mikroorganizmaların moleküler analizlerini PCR, klonlama, dizileme kıyaslaması, FISH, DGGE…) gerçekleştirebilirler.
2	Mikrobiyal toplulukların anahtar organizmalarını tanılayabilirler ve sayısallandırabilirler
3	Farklı moleküler ve mikrobiyolojik analizlerin kuvvetli ve zayıf yönlerini açıklayabilirler
4	Mikrobiyal ekolojide rastlanan problemlere moleküler mikrobiyal ekoloji kavramlarını uygulayabilirler

Mode of Delivery

Face to Face

Prerequisites and co-requisities

Recommended Optional Programme Components

None

Course Contents

 The "molecular revolution" in microbial ecology; microbial diversity; the rRNA approach  Recent strategies for the molecular identification and quantification of microbes and their activities in nature  Work with gene databases, clone libraries, and genetic fingerprints  Literature and web search for gene probes; probe design/evaluation

Weekly Detailed Course Contents

Week	Theoretical	Practice	Laboratory
1	Çevresel Örneklerde Nükleik asit ekstraksiyonu
2	Prokaryotik sistematiği: 16S rRNA PCR’ı and dizileme analizleri
3	Prokaryotik sistematiği: 16S rRNA PCR’ı and dizileme analizleri
4	Jenerasyon ve klon kütüphane analizleri
5	Klon kütüphanelerinin kantitatif analizler ve coverage
6	Klon kütüphanelerinin kantitatif analizler ve coverage
7	arasınav
8	Filogenetik inference
9	Filogenetik inference
10	Farklı moleküler tekniklere kıyasla filogenetik analizler
11	Mikrobiyal toplulukların DNA parmak izi
12	Çevresel izolatların moleküler tiplendirmesi
13	Çevresel izolatların moleküler tiplendirmesi
14	Çevresel izolatların moleküler tiplendirmesi
15	Çevresel izolatların moleküler tiplendirmesi
16	Final

Recommended or Required Reading

1- Molecular microbial ecology. Mark Osborn and Cindy Smith (eds). Published/Created: New York, NY : Taylor & Francis, c2005. 2- Madigan, M.T., Bender, K.S., Buckley, D.H., Sattley, W.M., Stahl, D.A., "Brock Biology of Microorganisms (15th Edition)"Benjamin Cummings, 2018.

Planned Learning Activities and Teaching Methods

Activities are given in detail in the sections of "Assessment Methods and Criteria" and "Workload Calculation".

Assessment Methods and Criteria

Term (or Year) Learning Activities	Quantity	Weight
Midterm Examination	1	100
SUM		100
End Of Term (or Year) Learning Activities	Quantity	Weight
Final Examination	1	100
SUM		100
Term (or Year) Learning Activities		40
End Of Term (or Year) Learning Activities		60
SUM		100

Work Placement(s)

None

Workload Calculation

Activities	Number	Time (hours)	Total Work Load (hours)
Midterm Examination	14	3	42
Final Examination	1	2	2
Team/Group Work	14	2	28
Report Preparation	1	5	5
Self Study	30	2	60
Individual Study for Mid term Examination	4	8	32
Individual Study for Final Examination	5	8	40
Reading	15	2	30
TOTAL WORKLOAD (hours)			239

Contribution of Learning Outcomes to Programme Outcomes

	PO 1	PO 2	PO 3	PO 4	PO 5	PO 6	PO 7
LO1
LO2
LO3
LO4

* Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High